Kidney Proximal Tubule [Weber et al. 2016]

Kidney PT (UW)

Center University of Washington Kidney Research Institute (UWKRI)
Institution University of Washington
Model Type Fluidic-3D
Device Nortis Par-V1
Organ Kidney
Model Image
Device Image
Description An initial version of the KPT MPS model, based on Nortis' prototype Par-V1 device. This model was used in the Weber et al. 2016

The kidney tubule MPS exhibits long-term viability, retains polarized expression and function of proteins essential for reabsorptive and secretory transport, responds to physiological stimuli, and performs critical biochemical synthetic activities.

PTECs grown in the MPS polarize with proteins selectively localized to the basolateral and apical aspects of the tubular epithelium and exhibit expected morphological and functional phenotypes of proximal tubule epithelium in vivo out to 28+ days.
References Weber EJ, Chapron A, Chapron BD, Voellinger JL, Lidberg KA, Yeung CK, Wang Z, Yamaura Y, Hailey DW, Neumann T, Shen DD, Thummel KE, Muczynski KA, Himmelfarb J, Kelly EJ. Development of a microphysiological model of human kidney proximal tubule function.. Kidney Int.. 2016. doi:10.1016/j.kint.2016.06.011. PMID:27521113
Model Region Dimensions Unspecified
Locations
Location Description
Influent Inflow media or buffer
Chamber Central chamber of device. Location of cells added to form KPT
Effluent-Apical Outflow media which had flowed in the apical side of the formed KPT
Cells
proximal tubule epithelial (Human Kidney)
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