Target/Analyte:
Cell Morphology

Short Name Cell Morphology
Description Imaging, typically with transmitted light, to see the cell morphology.

Method/Kits with this Target/Analyte
Method/Kit Description
Live imaging of cells stained with calcein/AM and draq7 Cells in culture are stained with calcein/AM and draq 7 and then imaged by transmission and fluorescence microscopy, to asses the morphology and live/dead cell number and distribution in live cultures (2D and 3D)
Confocal Microscopy Leica SP5 Inverted Confocal Microscope. Excitation frequencies used: 405, 488, 633
Immunocytochemistry and immunofluorescence protocol Immunocytochemistry (ICC) combines anatomical, immunological and biochemical techniques to image discrete components in tissues by using appropriately-labeled antibodies to bind specifically to their target antigens in situ.
Immunocytochemistry and immunofluorescence protocol (DAPI, FITC, Cy5) Immunocytochemistry (Cy5, DAPI, FITC) used for imaging and observation of HO-1, DNA, OAT4.
Immunocytochemistry and immunofluorescence protocol (DAPI, FITC, TRITC), Phase Contrast DNA (DAPI, blue), Neutral Lipids (FITC, green), Mitochondria (TRITC, yellow), Tissue Structure (unlabeled, phase contrast)
Immunohistochemistry (DAPI, Alexa Fluor-488, Alexa Fluor-568), Transmitted Light Immunohistochemistry (IHC) - imaging antigens (proteins) in cells of a tissue section based on antibodies binding to antigens tissues. Combination of four targets.
Microscopy - Brightfield Microscopy using brightfield sample illumination
Microscopy - Confocal 405, 488, 633
Microscopy - Fluorescence Imaging with some unspecified number of fluorescent marker and/or stains.
Microscopy - Phase Contrast Imaging with phase contrast only.

Studies Utilizing this Target/Analyte
Study Start Date Study Types Description
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