Target/Analyte:
COL1A2

Short Name COL1A2
Description Collagen Type I Alpha 2 Chain

Method/Kits with this Target/Analyte
Method/Kit Description
qRT-PCR Extraction and purification of RNA were carried out with a Qiagen RNeasy® fibrous tissue mini kit (Qiagen, Venlo, Netherlands) per manufacturer instructions. The RNA concentration yielded was determined by a QubitTM RNA HS assay Kit (Thermo Fisher, Waltham, MA). One hundred nanograms of RNA were used to synthesize cDNA using an iScript cDNA synthesis kit (Bio-Rad, Hercules, CA). Gene expression was analyzed by quantitative RT-PCR using a SYBR qPCR Supermix in 10 μl of the reaction solution.
Immunohistochemistry (DAPI, Alexa Fluor-488, Alexa Fluor-568) Immunohistochemistry (IHC) - imaging antigens (proteins) in cells of a tissue section based on antibodies binding to antigens tissues. Combination of three targets.
Immunohistochemistry (DAPI, Alexa Fluor-488, Alexa Fluor-568), Transmitted Light Immunohistochemistry (IHC) - imaging antigens (proteins) in cells of a tissue section based on antibodies binding to antigens tissues. Combination of four targets.

Studies Utilizing this Target/Analyte
Study Start Date Study Types Description
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