Target/Analyte:
ALP

Short Name ALP
Description PCR target

Method/Kits with this Target/Analyte
Method/Kit Description
TNF alpha Human ELISA Kit (Invitrogen: KHC3011) The Human Tumor Necrosis Factor alpha (TNF alpha, TNF) ELISA research-use-only kit is to be used for the quantitative determination of TNF alpha in samples (see sample types indicated) using 96-well plates and a microplate reader. The assay will recognize both natural and recombinant forms of this target.
Human alpha-Fetoprotein DuoSet (R&D Systems: DY1369) INTENDED USE: For the development of sandwich ELISAs to measure natural and recombinant human alpha Fetoprotein (α-Fetoprotein). The Reagent Diluent recommended may be suitable for most cell culture supernate, serum, and plasma samples. This kit contains sufficient materials to run ELISAs on at least fifteen 96 well plates.
RT-PCR (Applied Biosystems: StepOnePlus and SYBR Green Reaction Mix) Real-time PCR noted in Tuan work. RT-PCR (Applied Biosystems: StepOnePlus and SYBR Green Reaction Mix) Lin et al. Mol. Pharmaceutics 2014) The SYBR Green I dye chemistry uses Applied Biosystems™ SYBR™ Green I dye, a highly specific, double-stranded DNA binding dye, to detect PCR product as it accumulates during PCR cycles. The most important difference between the TaqMan and SYBR Green I dye chemistries is that the SYBR Green I dye chemistry will detect all double-stranded DNA, including non-specific reaction products. A well-optimized reaction is therefore essential for accurate results.
ProcartaPlex assay Cytokine detection assay
Luminex Human Discovery Assay (8-Plex) LXSAHM-08 The Luminex Assay is a bead-based immunoassay multiplex technique that allows multiple analytes (ex. cytokines, chemokines, metabolic biomarkers, etc.) to be quantitated simultaneously in a single biological matrix sample. Generally, the results obtained using a standardized Luminex assay are superior or equivalent to conventional ELISA, even as the differentiated xMAP technology allows high throughput biomarker testing with reduced sample volume, test preparation, and reagent costs. Luminex immunoassays can multiplex up to 100 compatible analytes simultaneously using a commercial or custom kit. The 8-Plex quantitates 8 analytes simultaneously and was used for the hToc experiment at CMSR @ UR. CMSR @ UR chose the 8 cytokines (Monocyte Chemoattractant Protein-1, Interleukin 6, C-C Motif Chemokine Ligand 3, Interleukin 10, C-X-C Motif Chemokine Ligand 10, Interleukin 1 beta, Tumor necrosis Factor alpha, Interleukin 17) to test the cell supernatant of our top well at different time points and culture conditions.
Unspecified Not specified by the user
Human alpha-Fetoprotein Quantikine ELISA Kit (R&D System; DAFP00) The Quantikine Human AFP immunoassay is a 4.5 hour solid phase ELISA designed to measure human AFP in cell culture supernates, serum, and plasma. https://www.rndsystems.com/products/human-alpha-fetoprotein-quantikine-elisa-kit_dafp00
qRT-PCR Extraction and purification of RNA were carried out with a Qiagen RNeasy® fibrous tissue mini kit (Qiagen, Venlo, Netherlands) per manufacturer instructions. The RNA concentration yielded was determined by a QubitTM RNA HS assay Kit (Thermo Fisher, Waltham, MA). One hundred nanograms of RNA were used to synthesize cDNA using an iScript cDNA synthesis kit (Bio-Rad, Hercules, CA). Gene expression was analyzed by quantitative RT-PCR using a SYBR qPCR Supermix in 10 μl of the reaction solution.
Luminex xMAP A bead-based multiplexed immunoassay system in a microplate format
Quantitative PCR (ThermoFisher) QuantStudio 12K Flex Real-Time PCR System with SYBR Green Master Mix
Human TNF-Alpha ELISA Kit (ThermoFisher: EH3TNFA) Assay TNFα (human tumor necrosis factor alpha) levels in human serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Human TNF alpha ELISA Kit.
Fluorescent quantification (ImageJ, Measure tool) Mean fluorescence per field, measured in ImageJ with the measure tool.
Mouse TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine (Boster: EK0527) ouse TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. Cited in 290 publication(s). Independently reviewed in 3 review(s).
Immunocytochemistry and immunofluorescence protocol (DAPI, FITC, Cy5) Immunocytochemistry (Cy5, DAPI, FITC) used for imaging and observation of HO-1, DNA, OAT4.

Studies Utilizing this Target/Analyte
Study Start Date Study Types Description
Loading...