Target/Analyte:
DNA

Short Name DNA
Description Immunocytochemistry imaging.

Method/Kits with this Target/Analyte
Method/Kit Description
Dual label immunofluorescence with DAPI and phalloidin counterstain Fixed cells are permeabilized with triton X-100 and incubated in blocking solution (depending on the antibodies used), then stained with Alexa Fluor 647 phalloidin (concentration optimized per cell line) for 2 hrs. at room temperature, followed by overnight incubation at 4°C with a mixture of two primary antibodies (concentration optimized per cell line and target) and then by 1 hr. incubation at room temperature with a secondary antibody mixture (concentration optimized depending on target and sample). Finally cells are stained with DAPI (1 µg/ml) for 10 min at room temperature. Final processing depends on the nature of the sample, tissue sections, cells on coverslips, transwell membrane fragments, are mounted (mounting medium depending on the sample) on microscope slides. Cells grown on multiwell plates (including mimetas plates) are images directly after the final wash. Imaging performed by fluorescence microscopy (widefield or confocal)
Immunocytochemistry and immunofluorescence protocol Immunocytochemistry (ICC) combines anatomical, immunological and biochemical techniques to image discrete components in tissues by using appropriately-labeled antibodies to bind specifically to their target antigens in situ.
Immunohistochemistry (DAPI, Alexa Fluor-488, Alexa Fluor-568), Transmitted Light Immunohistochemistry (IHC) - imaging antigens (proteins) in cells of a tissue section based on antibodies binding to antigens tissues. Combination of four targets.
Immunocytochemistry and immunofluorescence protocol (DAPI, FITC, Cy5), Phase Contrast DNA (DAPI, blue), OAT4 in Cells (FITC, green), HO-1 in Cells (Cy5, red), Tissue Structure (unlabeled, phase contrast)
Immunocytochemistry and immunofluorescence protocol (DAPI, FITC, Cy5) Immunocytochemistry (Cy5, DAPI, FITC) used for imaging and observation of HO-1, DNA, OAT4.
Immunocytochemistry and immunofluorescence protocol (DAPI, FITC, TRITC) DNA (DAPI, blue), Neutral Lipids (FITC, green), Mitochondria (TRITC, yellow)
Immunocytochemistry and immunofluorescence protocol (DAPI) ICC using the DAPI stain
Immunocytochemistry and immunofluorescence protocol (DAPI, Cy5) ICC of DAPI and Cy5
Dual label immunofluorescence with DAPI and phalloidin counterstain Fixed cells are permeabilized with triton X-100 and incubated in blocking solution (depending on the antibodies used), then stained with Alexa Fluor 647 phalloidin (concentration optimized per cell line) for 2 hrs. at room temperature, followed by overnight incubation at 4°C with a mixture of two primary antibodies (concentration optimized per cell line and target) and then by 1 hr. incubation at room temperature with a secondary antibody mixture (concentration optimized depending on target and sample). Finally cells are stained with DAPI (1 µg/ml) for 10 min at room temperature. Final processing depends on the nature of the sample, tissue sections, cells on coverslips, transwell membrane fragments, are mounted (mounting medium depending on the sample) on microscope slides. Cells grown on multiwell plates (including mimetas plates) are images directly after the final wash. Imaging performed by fluorescence microscopy (widefield or confocal)
Immunocytochemistry and immunofluorescence protocol (DAPI, FITC, TRITC), Phase Contrast DNA (DAPI, blue), Neutral Lipids (FITC, green), Mitochondria (TRITC, yellow), Tissue Structure (unlabeled, phase contrast)

Studies Utilizing this Target/Analyte
Study Start Date Study Types Description
Loading...