Target/Analyte:
TNNT2 Gene Expression

Short Name TNNT2
Description Expression levels of genes of interest were first calculated relative to expression level of a reference human hypoxanthine hosphoribosyltransferase-1 (HPRT-1) gene. These relative expression levels in IFNγ -treated samples were then shown normalized to corresponding relative expression levels in untreated control samples.

Method/Kits with this Target/Analyte
Method/Kit Description
qRT-PCR Extraction and purification of RNA were carried out with a Qiagen RNeasy® fibrous tissue mini kit (Qiagen, Venlo, Netherlands) per manufacturer instructions. The RNA concentration yielded was determined by a QubitTM RNA HS assay Kit (Thermo Fisher, Waltham, MA). One hundred nanograms of RNA were used to synthesize cDNA using an iScript cDNA synthesis kit (Bio-Rad, Hercules, CA). Gene expression was analyzed by quantitative RT-PCR using a SYBR qPCR Supermix in 10 μl of the reaction solution.

Studies Utilizing this Target/Analyte
Study Start Date Study Types Description
Loading...