Target/Analyte:
Nuclei

Short Name Nuclei
Description Nuclei as observed by image staining.

Method/Kits with this Target/Analyte
Method/Kit Description
Biosensor General Nuclear Biosensor
Confocal Microscope Fluorescence images were acquired using an Andor Dragonfly spinning disk confocal microscope at ×10 to ×40 magnification
Confocal Microscopy Leica SP5 Inverted Confocal Microscope. Excitation frequencies used: 405, 488, 633
Hoechst 33342 Staining Hoechst 33342 is a commonly used cell-permeant blue fluorescent compound that binds and stains cellular DNA.
Lonza AdipoRed Assay Reagent (Lonza: PT-7009), Hoechst 33342 Combined assay and stain. The AdipoRedTM Assay Reagent is designed for assessing the effect of compounds on the differentiation of preadipocytes or on lipid utilization in mature adipocytes. The lipophilic AdipoRedTM Assay Reagent specifically partitions into the fat droplets of differentiated adipocytes and fluoresces at 572 nm. This objective, high-throughput, homogeneous fluorescence-based assay quantifies the accumulation of intracellular triglycerides and provides significant advantages to drug discovery efforts in the field of obesity and diabetes. It is more sensitive than other methods such as the Oil Red O assay, and is much faster and easier than Northern and Western blots (https://bioscience.lonza.com/lonza_bs/US/en/Cell-analysis/p/000000000000186733/AdipoRed-Assay-Reagent%2C-20-mL). And Hoechst 33342 to see the nucleus.
Propidium Iodide/Hoechst 33342 PI Stain
DAPI (Invitrogen: P36931), FITC-Phalloidin (Sigma: P5282) Combination of stains used on heart model.
Immunocytochemistry and immunofluorescence protocol Immunocytochemistry (ICC) combines anatomical, immunological and biochemical techniques to image discrete components in tissues by using appropriately-labeled antibodies to bind specifically to their target antigens in situ.
Immunocytochemistry and immunofluorescence protocol (DAPI, FITC, Cy5) Immunocytochemistry (Cy5, DAPI, FITC) used for imaging and observation of HO-1, DNA, OAT4.
Immunocytochemistry and immunofluorescence protocol (DAPI, FITC, TRITC) DNA (DAPI, blue), Neutral Lipids (FITC, green), Mitochondria (TRITC, yellow)
Immunohistochemistry (DAPI, Alexa Fluor-488, Alexa Fluor-568) Immunohistochemistry (IHC) - imaging antigens (proteins) in cells of a tissue section based on antibodies binding to antigens tissues. Combination of three targets.
LIVE/DEAD Viability/Cytotoxicity Kit for mammalian cells (Molecular Probes: 03224 - Calcein AM/EthD-1), Hoechst Live/Dead Imaging (Calcein AM, Hoechst, EthD-1) for live, dead, and model structure.
LIVE/DEAD Viability/Cytotoxicity Kit for mammalian cells (Molecular Probes: 03224 - Calcein AM/EthD-1), Hoechst, Phase Contrast Nucleus (Hoechst, blue), Live Cells (Calcein AM, green), Dead Cells (EthD-1, red), Tissue Structure (unlabeled, phase contrast)
Microscopy - Confocal 405, 488, 633
Microscopy - Fluorescence Imaging with some unspecified number of fluorescent marker and/or stains.

Studies Utilizing this Target/Analyte
Study Start Date Study Types Description
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