Target/Analyte:
Permeability - 10 μg/ml of FITC-IgG

Short Name 10 μg/ml of FITC-IgG
Description Serial fluorescence images with the same exposure time were taken every 5 min for up to 20 min.
Then the permeability of TEBVs was calculated using equation: P= r/2ΔI * (dI/dt)t=0,
where ΔI is the change in total fluorescence intensity upon addition of labeled molecule (t = 0)–Background signal; (dI/dt)t=0 is the initial rate of transport of fluorescent dextran from the vessel lumen into the vessel, which can be approximated as (dI/dt)t=0≈(It−I(t=0))/t, where It is the intensity outside the vessel at time t, It=0 is the intensity outside the vessel at time t = 0; r is the radius of the lumen, which is estimated from width/2 of the fluorescent region at t = 0.

Method/Kits with this Target/Analyte
Method/Kit Description
ImageJ The number of MB with calcein AM-positive live cells was quantified using ImageJ.

Studies Utilizing this Target/Analyte
Study Start Date Study Types Description
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