Target/Analyte:
CD36

Short Name CD36
Description For flow cytometry, monocytes were stained with fluorescently labeled antibodies, including PE anti-human CD80 (Biolegend, 1:50), APC anti-human CD36 (Biolegend, 1:50), Pacific Blue™ anti-human CD206 (BD Pharmingen, 1:25) or Pacific Blue™ anti-human CD14 (Biolegend, 1:50), for 20 min on ice. The APC Mouse IgG2a (Biolegend, 1:50), PE Mouse IgG1(Biolegend, 1:50), and Pacific Blue™ Mouse IgG1 (Biolegend. 1:50) were used to stain the cells as isotype control, which was used to set the selected gate during testing. Then stained cells were collected by FACSCanto (BD Bioscience) and analyzed by FlowJo (Treestar, MAC version, v9.3.2) software.

Method/Kits with this Target/Analyte
Method/Kit Description
Edu Click-iT and CD markers staining Cell proliferation, Cell viability and Myeloid Cell lineage differentiation Surface markers
Flow Cytometry Flow cytometry is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles.
ImageJ The number of MB with calcein AM-positive live cells was quantified using ImageJ.
qRT-PCR Extraction and purification of RNA were carried out with a Qiagen RNeasy® fibrous tissue mini kit (Qiagen, Venlo, Netherlands) per manufacturer instructions. The RNA concentration yielded was determined by a QubitTM RNA HS assay Kit (Thermo Fisher, Waltham, MA). One hundred nanograms of RNA were used to synthesize cDNA using an iScript cDNA synthesis kit (Bio-Rad, Hercules, CA). Gene expression was analyzed by quantitative RT-PCR using a SYBR qPCR Supermix in 10 μl of the reaction solution.

Studies Utilizing this Target/Analyte
Study Start Date Study Types Description
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