Target/Analyte:
Monocyte Accumulation Rate

Short Name Monocyte/cm^2
Description To measure monocyte accumulation and migration, monocytes were labeled with cell tracker red-CMTPX (Life Tech) before addition to the perfusion loop. At the end of monocyte perfusion, the TEBV lumen was washed with PBS gently to remove unattached cells. Then the TEBVs were fixed with 4% Paraformaldehyde (PFA) solution for one hour. The TEBV is opened en face and compressed between two coverslips. The accumulation of monocytes is analyzed based on fluorescence images and the migration of monocytes is analyzed from confocal images at different depths into the TEBV from the endothelial surface. For

Method/Kits with this Target/Analyte
Method/Kit Description
Microscopy - Fluorescence Imaging with some unspecified number of fluorescent marker and/or stains.

Studies Utilizing this Target/Analyte
Study Start Date Study Types Description
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