Target/Analyte:
Collagen I

Short Name Collagen I
Description Collagen I expression

Method/Kits with this Target/Analyte
Method/Kit Description
RT-PCR Total RNA was isolated from the entire TEBV directly using RNeasy Fibrous Tissue Mini Kit (QIAGEN). The purity and concentration of RNA were measured using a Nano-Drop Spectrophotometer. For reverse transcription of RNA into cDNA, 250 ng RNA from the entire TEBV is used for each sample. Reverse transcription was performed using the iScript cDNA Synthesis Kit (BioRad). The PCR reaction was mixed by following the protocol of the iQ SYBR Green Supermix (Bio-Rad). RT-PCR was performed using a CFX Connect Real-Time PCR Detection System (Bio-Rad).
Immunocytochemistry and immunofluorescence protocol Immunocytochemistry (ICC) combines anatomical, immunological and biochemical techniques to image discrete components in tissues by using appropriately-labeled antibodies to bind specifically to their target antigens in situ.
Immunocytochemistry and immunofluorescence protocol (DAPI, FITC, Cy5) Immunocytochemistry (Cy5, DAPI, FITC) used for imaging and observation of HO-1, DNA, OAT4.
Immunocytochemistry and immunofluorescence protocol (DAPI, FITC, TRITC) DNA (DAPI, blue), Neutral Lipids (FITC, green), Mitochondria (TRITC, yellow)

Studies Utilizing this Target/Analyte
Study Start Date Study Types Description
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