Target/Analyte:
Calcium Transients

Short Name VALA
Description Voltage analysis is performed using the CyteSeer image analysis software, which automatically recognizes Hoechst-stained nuclei, segments, and identifies each cell to quantify voltage data for each cell captured in the field of view. Cells are included if four voltage peaks are detected (first paced beat is only partially captured by the imaging system); cells with fewer than four peaks indicated inadequate capture, and cells with more than four peaks indicate that cells had a spontaneous beating rate that was faster than the paced beats. The peak rise time, CTD25 (25% duration of the calcium transient, or duration at 25% decline from maximum amplitude), CTD75 (75% duration of the calcium transient, or duration at 75% decline from maximum amplitude, T75-25 (time for voltage to decay from 75% to 25% of maximum), and downstroke velocity were quantified using the CyteSeer image analysis software.

Method/Kits with this Target/Analyte
Method/Kit Description
15V Stimulation Recording of the electrical response generated following a 15V electrical stimulation delivered through an exogenous field stimulating electrode.
Microscopy - Fluorescence Imaging with some unspecified number of fluorescent marker and/or stains.

Studies Utilizing this Target/Analyte
Study Start Date Study Types Description
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