Target/Analyte:
Cell Viability (Quantitative)

Short Name Viability
Description Measuring cell viability with a quantitative method like an LDH ELISA, or ATP with CellTiter-Glo

Method/Kits with this Target/Analyte
Method/Kit Description
alamarBlue Cell Viability Reagent (Thermo: DAL1100) alamarBlue Cell Viability Reagent is a ready-to-use resazurin-based solution that functions as a cell health indicator by using the reducing power of living cells to quantitatively measure viability
Calcein, AM C1430 Calcein AM is a cell-permeant dye that can be used to determine cell viability in most eukaryotic cells. In live cells the nonfluorescent calcein AM is converted to a green-fluorescent calcein after acetoxymethyl ester hydrolysis by intracellular esterases.
CellTiter-Glo Luminescent Cell Viability Assay (Promega: G7573) The CellTiter-Glo® Luminescent Cell Viability Assay is a homogeneous method to determine the number of viable cells in culture based on quantitation of the ATP present, which signals the presence of metabolically active cells. The CellTiter-Glo® Assay is designed for use with multiwell-plate formats, making it ideal for automated high-throughput screening (HTS) and cell proliferation and cytotoxicity assays. The homogeneous assay procedure involves adding a single reagent (CellTiter-Glo® Reagent) directly to cells cultured in serum-supplemented medium. Cell washing, removal of medium or multiple pipetting steps are not required.
LDH Cytotoxicity Assay LDH Cytotoxicity Assay (ab65393)
MTT (McAleer et al. 2019) MTT powder was dissolved in growth medium to a final concentration of 5 mg/ml. Cells were incubated in 500 μl of the solution for 90 min at 37°C and 5% CO2. The medium was removed, and resultant crystals were dissolved in a lysis buffer consisting of 10% SDS with 0.5% acetic acid in dimethyl sulfoxide. The solution (100 μl) was placed into a 96-well plate and absorbance read at 570 nm using a BioTek Synergy HT plate reader (BioTek).
RealTime-Glo™ MT Cell Viability Assay (G9711, G9712, G9713) In the RealTime Glo MT Cell Viability Assay, a non-lytic NanoLuc Luciferase reaction occurs in the culture medium. NanoLuc Luciferase and MT Cell Viability Substrate are added to cell culture media. The MT Cell Viability Substrate then diffuses into cells where it is reduced to form a NanoLuc Substrate, which exits the cell and is used rapidly by NanoLuc Luciferase in the media. Only metabolically active cells can reduce the substrate, and light production is proportional to the number of live cells in culture. Dead cells cannot reduce the substrate.
Total Protein Assay Measured total protein content in cell suspension on Day 0 and from cultured cells in 2D and 3D on last day of culture, and calculated cell viability using the protein content on Day 0 (normalized to cell count) as 100% cell viability.
RealTime-Glo™ MT Cell Viability Assay (G9711, G9712, G9713) In the RealTime Glo MT Cell Viability Assay, a non-lytic NanoLuc Luciferase reaction occurs in the culture medium. NanoLuc Luciferase and MT Cell Viability Substrate are added to cell culture media. The MT Cell Viability Substrate then diffuses into cells where it is reduced to form a NanoLuc Substrate, which exits the cell and is used rapidly by NanoLuc Luciferase in the media. Only metabolically active cells can reduce the substrate, and light production is proportional to the number of live cells in culture. Dead cells cannot reduce the substrate.

Studies Utilizing this Target/Analyte
Study Start Date Study Types Description
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