Target/Analyte:
MMP-3 Expression normalized to 18S rRNA

Short Name MMP-3 Exp
Description Real-time PCR. Catabolic gene.

Method/Kits with this Target/Analyte
Method/Kit Description
RT-PCR (Applied Biosystems: StepOnePlus and SYBR Green Reaction Mix) Real-time PCR noted in Tuan work. RT-PCR (Applied Biosystems: StepOnePlus and SYBR Green Reaction Mix) Lin et al. Mol. Pharmaceutics 2014) The SYBR Green I dye chemistry uses Applied Biosystems™ SYBR™ Green I dye, a highly specific, double-stranded DNA binding dye, to detect PCR product as it accumulates during PCR cycles. The most important difference between the TaqMan and SYBR Green I dye chemistries is that the SYBR Green I dye chemistry will detect all double-stranded DNA, including non-specific reaction products. A well-optimized reaction is therefore essential for accurate results.

Studies Utilizing this Target/Analyte
Study Start Date Study Types Description
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