Target/Analyte:
Osteopontin

Short Name OPN
Description Osteopontin (OPN) is a 35kDa secreted adhesive glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein family (SIBLING) and it is expressed mainly in bone, teeth, kidney, epithelial lining tissues, activated T cells and macrophages and found in plasma and breast milk.

It is processed by matrix metalloproteases 3 and 4 as well as thrombin, which generate two functional fragments: an N terminal (RGD) fragment that binds to integrin receptors and a C terminal fragment (containing heparin binding sites) that interacts with CD44. Osteopontin can be present as a cytokine-like soluble protein or an immobilized extracellular matrix protein.

Osteopontin is important in multiple physiologic and pathologic processes including survival, adhesion, migration, angiogenesis, apoptosis, inflammation, macrophage migration, B cell proliferation, Ig production, wound healing and tumor metastasis.

Method/Kits with this Target/Analyte
Method/Kit Description
Human Bone Panel II Kit (MSD: K15147C) The Human Bone Marker Panel II Kit provides assay-specific components for the quantitative determination of both natural and recombinant human osteocalcin (OCL), osteonectin (ONN), and osteopontin (OPN) in serum and plasma.
Human Osteopontin Quantikine ELISA (R&D Systems DOST00) Human osteopontin secretion ELISA
Osteopontin Human SimpleStep ELISA Kit (abcam: ab192143) Osteopontin in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Osteopontin protein in human serum, plasma, milk, urine and cell culture supernatant.
Quantitative PCR (ThermoFisher) QuantStudio 12K Flex Real-Time PCR System with SYBR Green Master Mix

Studies Utilizing this Target/Analyte
Study Start Date Study Types Description
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