Target/Analyte:
Cytokeratin-18

Short Name CK-18
Description EMT marker.

Method/Kits with this Target/Analyte
Method/Kit Description
Cytokeratin-18 Staining Immunocytochemical staining for cytokeratin-18 (1 μl/mL; ab668). Cells were fixed with 4% paraformaldehyde for 15 min, permeabilized with 0.5% Triton X for 10 min, and blocked with 3% bovine serum albumin in PBS for 30 min prior to incubation with primary antibodies overnight at 4°C. After washing with PBS 3 times 5 min each, devices were incubated with Alexa Fluor 488- and 594-conjugated secondary antibodies (Thermo Fisher Scientific) and diluted 1:1000 in 3% bovine serum albumin for 1 h in the dark. The devices were washed with PBS 3 times 5 min each, treated with NucBlue Live ReadyProbes Reagent (Thermo Fisher Scientific).
Cytokeratin-18 Staining Immunocytochemical staining for cytokeratin-18 (1 μl/mL; ab668). Cells were fixed with 4% paraformaldehyde for 15 min, permeabilized with 0.5% Triton X for 10 min, and blocked with 3% bovine serum albumin in PBS for 30 min prior to incubation with primary antibodies overnight at 4°C. After washing with PBS 3 times 5 min each, devices were incubated with Alexa Fluor 488- and 594-conjugated secondary antibodies (Thermo Fisher Scientific) and diluted 1:1000 in 3% bovine serum albumin for 1 h in the dark. The devices were washed with PBS 3 times 5 min each, treated with NucBlue Live ReadyProbes Reagent (Thermo Fisher Scientific).
Immunocytochemistry and immunofluorescence protocol (DAPI, FITC, Cy5) Immunocytochemistry (Cy5, DAPI, FITC) used for imaging and observation of HO-1, DNA, OAT4.
Immunofluorescence indirect or direct immunofluorescence staining
Microscopy - Confocal 405, 488, 633

Studies Utilizing this Target/Analyte
Study Start Date Study Types Description
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