Target/Analyte:
MCP-1

Short Name MCP-1
Description Monocyte Chemoattractant Protein-1, is a key chemokine that regulates
migration and infiltration of monocytes/macrophages.

Method/Kits with this Target/Analyte
Method/Kit Description
Luminex Human Discovery Assay (8-Plex) LXSAHM-08 The Luminex Assay is a bead-based immunoassay multiplex technique that allows multiple analytes (ex. cytokines, chemokines, metabolic biomarkers, etc.) to be quantitated simultaneously in a single biological matrix sample. Generally, the results obtained using a standardized Luminex assay are superior or equivalent to conventional ELISA, even as the differentiated xMAP technology allows high throughput biomarker testing with reduced sample volume, test preparation, and reagent costs. Luminex immunoassays can multiplex up to 100 compatible analytes simultaneously using a commercial or custom kit. The 8-Plex quantitates 8 analytes simultaneously and was used for the hToc experiment at CMSR @ UR. CMSR @ UR chose the 8 cytokines (Monocyte Chemoattractant Protein-1, Interleukin 6, C-C Motif Chemokine Ligand 3, Interleukin 10, C-X-C Motif Chemokine Ligand 10, Interleukin 1 beta, Tumor necrosis Factor alpha, Interleukin 17) to test the cell supernatant of our top well at different time points and culture conditions.
Luminex Performance Human XL Cytokine Panel (6-Plex) FCSTM18B-06 Assays were completed according to the manufacturer’s instructions at The University of Pittsburgh Cancer Proteomics Facility Luminex® Core Laboratory. All multiplex panels were run at the same time to avoid run-to-run and operator error variability utilizing the xMAP platform licensed by Luminex®. All the cytokine target (CCL2/JE/MCP-1, IL-1 beta/IL-1F2, IL-6, IL-8/CXCL8, IL-10, TNF-alpha) profiling experiments were performed from efflux obtained from devices for each experimental condition. (https://www.rndsystems.com/products/human-xl-cytokine-luminex-performance-panel-premixed-kit_fcstm18b)

Studies Utilizing this Target/Analyte
Study Start Date Study Types Description
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