Target/Analyte:
FGF2

Short Name FGF2
Description FGF basic/FGF2/bFGF

Method/Kits with this Target/Analyte
Method/Kit Description
qRT-PCR Extraction and purification of RNA were carried out with a Qiagen RNeasy® fibrous tissue mini kit (Qiagen, Venlo, Netherlands) per manufacturer instructions. The RNA concentration yielded was determined by a QubitTM RNA HS assay Kit (Thermo Fisher, Waltham, MA). One hundred nanograms of RNA were used to synthesize cDNA using an iScript cDNA synthesis kit (Bio-Rad, Hercules, CA). Gene expression was analyzed by quantitative RT-PCR using a SYBR qPCR Supermix in 10 μl of the reaction solution.
V-PLEX Proinflammatory Panel 1 Human Kit (K15049D-1) he Proinflammatory Panel 1 (human) measures 10 cytokines that are important in inflammation response and immune system regulation as well as numerous other biological processes. These assays can detect secreted biomarkers in a variety of tissues and body fluids where over- or under-expression may indicate a shift in biological equilibrium. This panel also includes assays for many of the Th1/Th2 pathway biomarkers. The Proinflammatory Panel 1 (human) measures biomarkers that are implicated in a number of disorders, including rheumatoid arthritis, Alzheimer’s disease, asthma, atherosclerosis, allergies, systemic lupus erythematosus, obesity, cancer, depression, multiple sclerosis, diabetes, psoriasis, and Crohn’s disease. The Proinflammatory Panel 1 (human) Kit provides assay-specific components for the quantitative determination of IFN-γ, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, and TNF-α. This kit may be used for the analysis of all or any combination of these cytokine biomarkers in cell culture supernatants, serum, plasma, and urine.

Studies Utilizing this Target/Analyte
Study Start Date Study Types Description
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