Target/Analyte:
EGF

Short Name EGF
Description EGF

Method/Kits with this Target/Analyte
Method/Kit Description
Single cell migration count Statistics on the migration velocity of cells
V-PLEX Cytokine Panel 1 Human Kit (MSD: K15050D) The Cytokine Panel 1 (human) measures 10 cytokines that are important in inflammation response and immune system regulation as well as numerous other biological processes. These assays can detect secreted biomarkers in a variety of tissues and body fluids where over- or under-expression may indicate a shift in biological equilibrium. The Cytokine Panel 1 (human) Kit provides assay-specific components for the quantitative determination of GM-CSF, IL-1α, IL-5, IL-7, IL-12/IL-23p40, IL-15, IL-16, IL-17A, TNF-β, and VEGF-A. This kit may be used for the analysis of all or any combination of these cytokine biomarkers in cell culture supernatants, serum, plasma, and urine.
Luminex MAGPIX Masurement of secreted protein
qRT-PCR Extraction and purification of RNA were carried out with a Qiagen RNeasy® fibrous tissue mini kit (Qiagen, Venlo, Netherlands) per manufacturer instructions. The RNA concentration yielded was determined by a QubitTM RNA HS assay Kit (Thermo Fisher, Waltham, MA). One hundred nanograms of RNA were used to synthesize cDNA using an iScript cDNA synthesis kit (Bio-Rad, Hercules, CA). Gene expression was analyzed by quantitative RT-PCR using a SYBR qPCR Supermix in 10 μl of the reaction solution.
V-PLEX Proinflammatory Panel 1 Human Kit (K15049D-1) he Proinflammatory Panel 1 (human) measures 10 cytokines that are important in inflammation response and immune system regulation as well as numerous other biological processes. These assays can detect secreted biomarkers in a variety of tissues and body fluids where over- or under-expression may indicate a shift in biological equilibrium. This panel also includes assays for many of the Th1/Th2 pathway biomarkers. The Proinflammatory Panel 1 (human) measures biomarkers that are implicated in a number of disorders, including rheumatoid arthritis, Alzheimer’s disease, asthma, atherosclerosis, allergies, systemic lupus erythematosus, obesity, cancer, depression, multiple sclerosis, diabetes, psoriasis, and Crohn’s disease. The Proinflammatory Panel 1 (human) Kit provides assay-specific components for the quantitative determination of IFN-γ, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, and TNF-α. This kit may be used for the analysis of all or any combination of these cytokine biomarkers in cell culture supernatants, serum, plasma, and urine.
Luminex xMAP A bead-based multiplexed immunoassay system in a microplate format
Quantitative PCR (ThermoFisher) QuantStudio 12K Flex Real-Time PCR System with SYBR Green Master Mix

Studies Utilizing this Target/Analyte
Study Start Date Study Types Description
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