Target/Analyte:
IL-10

Short Name IL-10
Description Senescence Associated Secretory Phenotype (SASP) protein

Method/Kits with this Target/Analyte
Method/Kit Description
Luminex Human Discovery Assay (8-Plex) LXSAHM-08 The Luminex Assay is a bead-based immunoassay multiplex technique that allows multiple analytes (ex. cytokines, chemokines, metabolic biomarkers, etc.) to be quantitated simultaneously in a single biological matrix sample. Generally, the results obtained using a standardized Luminex assay are superior or equivalent to conventional ELISA, even as the differentiated xMAP technology allows high throughput biomarker testing with reduced sample volume, test preparation, and reagent costs. Luminex immunoassays can multiplex up to 100 compatible analytes simultaneously using a commercial or custom kit. The 8-Plex quantitates 8 analytes simultaneously and was used for the hToc experiment at CMSR @ UR. CMSR @ UR chose the 8 cytokines (Monocyte Chemoattractant Protein-1, Interleukin 6, C-C Motif Chemokine Ligand 3, Interleukin 10, C-X-C Motif Chemokine Ligand 10, Interleukin 1 beta, Tumor necrosis Factor alpha, Interleukin 17) to test the cell supernatant of our top well at different time points and culture conditions.
Luminex Performance Human XL Cytokine Panel (6-Plex) FCSTM18B-06 Assays were completed according to the manufacturer’s instructions at The University of Pittsburgh Cancer Proteomics Facility Luminex® Core Laboratory. All multiplex panels were run at the same time to avoid run-to-run and operator error variability utilizing the xMAP platform licensed by Luminex®. All the cytokine target (CCL2/JE/MCP-1, IL-1 beta/IL-1F2, IL-6, IL-8/CXCL8, IL-10, TNF-alpha) profiling experiments were performed from efflux obtained from devices for each experimental condition. (https://www.rndsystems.com/products/human-xl-cytokine-luminex-performance-panel-premixed-kit_fcstm18b)
MILLIPLEX Human Cytokine/Chemokine/Growth Factor Panel A - UTMB multiplex cytokine analysis
Photonic Ring Resonator Flow-Based Biosensor Material is flowed through a microfluidics device containing a packaged Photonic Integrated Circuit (PIC) Biosensor for the detection of target proteins in complex matrices.
V-PLEX Proinflammatory Panel 1 Human Kit (K15049D-1) he Proinflammatory Panel 1 (human) measures 10 cytokines that are important in inflammation response and immune system regulation as well as numerous other biological processes. These assays can detect secreted biomarkers in a variety of tissues and body fluids where over- or under-expression may indicate a shift in biological equilibrium. This panel also includes assays for many of the Th1/Th2 pathway biomarkers. The Proinflammatory Panel 1 (human) measures biomarkers that are implicated in a number of disorders, including rheumatoid arthritis, Alzheimer’s disease, asthma, atherosclerosis, allergies, systemic lupus erythematosus, obesity, cancer, depression, multiple sclerosis, diabetes, psoriasis, and Crohn’s disease. The Proinflammatory Panel 1 (human) Kit provides assay-specific components for the quantitative determination of IFN-γ, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, and TNF-α. This kit may be used for the analysis of all or any combination of these cytokine biomarkers in cell culture supernatants, serum, plasma, and urine.

Studies Utilizing this Target/Analyte
Study Start Date Study Types Description
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