Target/Analyte:
Nucleus

Short Name Nucleus
Description Nucleus of a cell. Typically this target is used when describing an image with a nuclear stain

Method/Kits with this Target/Analyte
Method/Kit Description
Hematoxylin and Eosin (H&E) Staining (Baylor College of Medicine Protocol) Hematoxylin & Eosin Stain (H&E) for imaging of the nucleus, cytoplasm, and extracellular proteins. This H&E staining follows the protocol created by the Baylor College of Medicine for staining samples from their Bone MPS model.
Immunocytochemistry and immunofluorescence protocol Immunocytochemistry (ICC) combines anatomical, immunological and biochemical techniques to image discrete components in tissues by using appropriately-labeled antibodies to bind specifically to their target antigens in situ.
Cell Viability Imaging (Hoechst, Calcein, Propidium Iodine) Cellular imaging with Hoecst, Calcein AM, and PI
Hematoxylin and Eosin (H&E) Staining Hematoxylin & Eosin Stain (H&E) for imaging of the nucleus, cytoplasm, and extracellular proteins. This has no specific protocol for the staining
Hematoxylin and Eosin (H&E) Staining (Baylor College of Medicine Protocol) Hematoxylin & Eosin Stain (H&E) for imaging of the nucleus, cytoplasm, and extracellular proteins. This H&E staining follows the protocol created by the Baylor College of Medicine for staining samples from their Bone MPS model.
Immunocytochemistry and immunofluorescence protocol Immunocytochemistry (ICC) combines anatomical, immunological and biochemical techniques to image discrete components in tissues by using appropriately-labeled antibodies to bind specifically to their target antigens in situ.
Immunocytochemistry and immunofluorescence protocol (DAPI, FITC, Cy5) Immunocytochemistry (Cy5, DAPI, FITC) used for imaging and observation of HO-1, DNA, OAT4.
Immunofluorescence indirect or direct immunofluorescence staining
Live/Dead Imaging (Hoechst, CalceinAM, EthD-1) Imaging with Hoechst, CalceinAM, and EthD-1 markers
LIVE/DEAD Viability/Cytotoxicity Kit for mammalian cells (Molecular Probes: 03224 - Calcein AM/EthD-1), Hoechst Live/Dead Imaging (Calcein AM, Hoechst, EthD-1) for live, dead, and model structure.
LIVE/DEAD Viability/Cytotoxicity Kit for mammalian cells (Molecular Probes: 03224 - Calcein AM/EthD-1), Hoechst, Phase Contrast Nucleus (Hoechst, blue), Live Cells (Calcein AM, green), Dead Cells (EthD-1, red), Tissue Structure (unlabeled, phase contrast)
Lonza AdipoRed Assay Reagent (Lonza: PT-7009), Hoechst 33342 Combined assay and stain. The AdipoRedTM Assay Reagent is designed for assessing the effect of compounds on the differentiation of preadipocytes or on lipid utilization in mature adipocytes. The lipophilic AdipoRedTM Assay Reagent specifically partitions into the fat droplets of differentiated adipocytes and fluoresces at 572 nm. This objective, high-throughput, homogeneous fluorescence-based assay quantifies the accumulation of intracellular triglycerides and provides significant advantages to drug discovery efforts in the field of obesity and diabetes. It is more sensitive than other methods such as the Oil Red O assay, and is much faster and easier than Northern and Western blots (https://bioscience.lonza.com/lonza_bs/US/en/Cell-analysis/p/000000000000186733/AdipoRed-Assay-Reagent%2C-20-mL). And Hoechst 33342 to see the nucleus.

Studies Utilizing this Target/Analyte
Study Start Date Study Types Description
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