Target/Analyte:
CYP2C9

Short Name CYP2C9
Description Cytochrome P450 2C9 is an important cytochrome P450 enzyme with a major role in the oxidation of both xenobiotic and endogenous compounds.

[https://en.wikipedia.org/wiki/CYP1A1]

Method/Kits with this Target/Analyte
Method/Kit Description
P450-Glo™ CYP2C9 Assay System The P450-Glo™ CYP2C9 Assay provides a homogeneous, luminescent method for measuring cytochrome P450 CYP2C9 activity. The assay employs luminogenic P450 substrates that are derivatives of beetle luciferin, a substrate for luciferase enzymes. The derivatives are not substrates for luciferase but are converted by P450s to luciferin, which in turn reacts with luciferase to produce light that is directly proportional to the activity of the P450. This luminescent assay exhibits exquisite sensitivity, low background signals and broad dynamic range. It generates a "glow-type" luminescent signal, produced using derivatized luciferins as P450 substrates and a recombinant stabilized luciferase (Ultra-Glo™ Luciferase) coupled with a proprietary buffer system. The half-life of the luminescent output is greater than two hours, eliminating the need for luminometers with injectors and allowing batch plate processing. The luminescent format also eliminates the need for time-consuming analyses such as HPLC. The luciferin-based substrates are readily taken up by cells and rapidly converted into luciferin inside the cell, which reduces the incubation time required (typically 30–60 minutes). Other benefits of the P450-Glo™ CYP2C9 Assay include a formulation that minimizes the incidence of false positives due to inhibition of luciferase by analytes when screening for cytochrome P450 inhibitors. Z´ values greater than 0.8 are achieved in either 96- or 384-well plate formats and confer highly predictive results.
Unspecified Not specified by the user

Studies Utilizing this Target/Analyte
Study Start Date Study Types Description
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