Target/Analyte:
Dead Cells

Short Name DEAD
Description Number or percent of dead cells

Method/Kits with this Target/Analyte
Method/Kit Description
DRAQ7 Non-toxic plasma membrane impermeant nuclear dye that can only enter cells with a damaged plasma membrane or dead cells
Ethidium Homodimer-1 (EthD-1) The cell-impermeant viability indicator ethdium homodimer-1 (EthD-1) is a high-affinity nucleic acid stain that is weakly fluorescent until bound to DNA and emits red fluorescence (excitation/emission maxima ∼528/617).
LIVE/DEAD Cell Imaging Kit (ThermoFisher: R37601) The LIVE/DEAD® Cell Imaging Kit is a sensitive two-color fluorescence cell viability assay optimized for FITC and Texas Red® filters. Quick and easy to use, the kit allows discrimination between live and dead cells with two probes that measure recognized parameters of cytotoxicity and cell viability—intracellular esterase activity and plasma membrane integrity (https://www.thermofisher.com/order/catalog/product/R37601).
LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells (L3224) Calcein-AM and ethidium homodimer-1
LIVE/DEAD Viability/Cytotoxicity Kit for mammalian cells (Molecular Probes: 03224 - Calcein AM/EthD-1), Hoechst Live/Dead Imaging (Calcein AM, Hoechst, EthD-1) for live, dead, and model structure.
Cell Viability Imaging (Hoechst, Calcein, Propidium Iodine) Cellular imaging with Hoecst, Calcein AM, and PI
DRAQ7 Non-toxic plasma membrane impermeant nuclear dye that can only enter cells with a damaged plasma membrane or dead cells
Immunocytochemistry and immunofluorescence protocol Immunocytochemistry (ICC) combines anatomical, immunological and biochemical techniques to image discrete components in tissues by using appropriately-labeled antibodies to bind specifically to their target antigens in situ.
LIVE/DEAD Cell Imaging Kit (ThermoFisher: R37601) The LIVE/DEAD® Cell Imaging Kit is a sensitive two-color fluorescence cell viability assay optimized for FITC and Texas Red® filters. Quick and easy to use, the kit allows discrimination between live and dead cells with two probes that measure recognized parameters of cytotoxicity and cell viability—intracellular esterase activity and plasma membrane integrity (https://www.thermofisher.com/order/catalog/product/R37601).
Live/Dead Imaging (Hoechst, CalceinAM, EthD-1) Imaging with Hoechst, CalceinAM, and EthD-1 markers
LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells (L3224) Calcein-AM and ethidium homodimer-1
LIVE/DEAD Viability/Cytotoxicity Kit for mammalian cells (Molecular Probes: 03224 - Calcein AM/EthD-1), Hoechst Live/Dead Imaging (Calcein AM, Hoechst, EthD-1) for live, dead, and model structure.
LIVE/DEAD Viability/Cytotoxicity Kit for mammalian cells (Molecular Probes: 03224 - Calcein AM/EthD-1), Hoechst, Phase Contrast Nucleus (Hoechst, blue), Live Cells (Calcein AM, green), Dead Cells (EthD-1, red), Tissue Structure (unlabeled, phase contrast)

Studies Utilizing this Target/Analyte
Study Start Date Study Types Description
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