Target/Analyte:
Rise Time

Short Name Rise Time
Description Average time to reach maximum Ca2+ influx in cell beating.

Method/Kits with this Target/Analyte
Method/Kit Description
15V Stimulation Recording of the electrical response generated following a 15V electrical stimulation delivered through an exogenous field stimulating electrode.
EarlyTox Cardiotoxicity Kit (Molecular Devices: R8211) The assay kit can be used for characterizing the impact of pharmacological compounds on peak frequency (BPM), peak amplitude, and beat pattern and is designed to work with stem cell-derived cardiomyocytes or primary cardiomyocytes. Peak frequencies are determined from changes in intracellular Ca++ concentration monitored by the EarlyTox Cardiotoxicity Dye. During a single beating cycle, Ca++ is released into the cytoplasm after the sarcoplasmic reticulum is stimulated. Calcium binds with troponin activating the sarcomere, and the cell contracts. Synchronously, there is an increase in fluorescent signal as dye binds to free calcium in the cytosol. Cellular relaxation occurs on removal of calcium from the cytosol by calcium uptake pumps of the sarcoplasmic reticulum and by calcium exchange with extracellular fluid. The fluorescent signal decreases as calcium concentration decreases. As the cycle repeats, additional fluorescent peaks synchronous with beating are observed.

Studies Utilizing this Target/Analyte
Study Start Date Study Types Description
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