Target/Analyte:
Beat Rate

Short Name BEAT RATE
Description Beat rate (e.g. of cardiomyocytes). Normally determined through video quantification.

Method/Kits with this Target/Analyte
Method/Kit Description
Clampfit (Axon Instruments) [McAleer et al. 2019] Software and formula used to quantify raw MEA data in McAleer et al. 2019
EarlyTox Cardiotoxicity Kit (Molecular Devices: R8211) The assay kit can be used for characterizing the impact of pharmacological compounds on peak frequency (BPM), peak amplitude, and beat pattern and is designed to work with stem cell-derived cardiomyocytes or primary cardiomyocytes. Peak frequencies are determined from changes in intracellular Ca++ concentration monitored by the EarlyTox Cardiotoxicity Dye. During a single beating cycle, Ca++ is released into the cytoplasm after the sarcoplasmic reticulum is stimulated. Calcium binds with troponin activating the sarcomere, and the cell contracts. Synchronously, there is an increase in fluorescent signal as dye binds to free calcium in the cytosol. Cellular relaxation occurs on removal of calcium from the cytosol by calcium uptake pumps of the sarcoplasmic reticulum and by calcium exchange with extracellular fluid. The fluorescent signal decreases as calcium concentration decreases. As the cycle repeats, additional fluorescent peaks synchronous with beating are observed.
Electrophysiological Activity The built in MEA measures spontaneous beat rate of cells at each of the 64 electrodes in the 8x8 array.
Microscopy Video Quantification (Manual) Quantification of a video made either from a microscopy video camera or a combined sequence of microscopy images. Videos are quantified by manual review.
Microscopy Video Quantification (MotionGUI) Quantification of a video made either from a microscopy video camera or a combined sequence of microscopy images. Videos are quantified with the MotionGUI software developed by the Healy Lab at UC Berkeley
Microscopy - Phase Contrast Imaging with phase contrast only.

Studies Utilizing this Target/Analyte
Study Start Date Study Types Description
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