Target/Analyte:
TNF-Alpha

Short Name TNFA
Description Tumor necrosis factor (TNF, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons.

Method/Kits with this Target/Analyte
Method/Kit Description
Human TNF-Alpha ELISA Kit (ThermoFisher: EH3TNFA) Assay TNFα (human tumor necrosis factor alpha) levels in human serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Human TNF alpha ELISA Kit.
Luminex Human Discovery Assay (8-Plex) LXSAHM-08 The Luminex Assay is a bead-based immunoassay multiplex technique that allows multiple analytes (ex. cytokines, chemokines, metabolic biomarkers, etc.) to be quantitated simultaneously in a single biological matrix sample. Generally, the results obtained using a standardized Luminex assay are superior or equivalent to conventional ELISA, even as the differentiated xMAP technology allows high throughput biomarker testing with reduced sample volume, test preparation, and reagent costs. Luminex immunoassays can multiplex up to 100 compatible analytes simultaneously using a commercial or custom kit. The 8-Plex quantitates 8 analytes simultaneously and was used for the hToc experiment at CMSR @ UR. CMSR @ UR chose the 8 cytokines (Monocyte Chemoattractant Protein-1, Interleukin 6, C-C Motif Chemokine Ligand 3, Interleukin 10, C-X-C Motif Chemokine Ligand 10, Interleukin 1 beta, Tumor necrosis Factor alpha, Interleukin 17) to test the cell supernatant of our top well at different time points and culture conditions.
Luminex xMAP A bead-based multiplexed immunoassay system in a microplate format
Mouse TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine (Boster: EK0527) ouse TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. Cited in 290 publication(s). Independently reviewed in 3 review(s).
Quantitative PCR (ThermoFisher) QuantStudio 12K Flex Real-Time PCR System with SYBR Green Master Mix
TNF alpha Human ELISA Kit (Invitrogen: KHC3011) The Human Tumor Necrosis Factor alpha (TNF alpha, TNF) ELISA research-use-only kit is to be used for the quantitative determination of TNF alpha in samples (see sample types indicated) using 96-well plates and a microplate reader. The assay will recognize both natural and recombinant forms of this target.
Unspecified Not specified by the user

Studies Utilizing this Target/Analyte
Study Start Date Study Types Description
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