Method/Kit:
Cell Proliferation Kit II (XTT) (Sigma: 11465015001)

Supplier Sigma-Aldrich
Measurement Type Absorbance
Description The Cell Proliferation Kit II (XTT) is a colorimetric assay for the nonradioactive quantification of cellular proliferation, viability, and cytotoxicity. Sample material is either adherent or suspension cells cultured in 96-well microplates.

In contrast to MTT, the cleavage product of XTT is soluble in water; therefore, a solubilization step is not required. The tetrazolium salt XTT is cleaved to formazan by a complex cellular mechanism. This bioreduction occurs in viable cells only, and is related to NAD(P)H production through glycolysis. Therefore, the amount of formazan dye formed directly correlates to the number of metabolically active cells in the culture.

(Sigma)


Target/Analytes with this Method/Kit
Target/Analyte Short Name Description
Tumor Growth TGrwth The change in the size of a measured tumor

Studies Utilizing this Method/Kit
Study Start Date Study Types Description
Loading...