Method/Kit:
Lonza AdipoRed Assay Reagent (Lonza: PT-7009), Hoechst 33342

Supplier Lonza
Measurement Type Fluorescence Intensity
Description Combined assay and stain. The AdipoRedTM Assay Reagent is designed for assessing the effect of compounds on the differentiation of preadipocytes or on lipid utilization in mature adipocytes. The lipophilic AdipoRedTM Assay Reagent specifically partitions into the fat droplets of differentiated adipocytes and fluoresces at 572 nm.

This objective, high-throughput, homogeneous fluorescence-based assay quantifies the accumulation of intracellular triglycerides and provides significant advantages to drug discovery efforts in the field of obesity and diabetes. It is more sensitive than other methods such as the Oil Red O assay, and is much faster and easier than Northern and Western blots (https://bioscience.lonza.com/lonza_bs/US/en/Cell-analysis/p/000000000000186733/AdipoRed-Assay-Reagent%2C-20-mL).

And Hoechst 33342 to see the nucleus.


Target/Analytes with this Method/Kit
Target/Analyte Short Name Description
Lipid to Nuclei Ratio LD:Nuc A comparison between quantified Lipid Droplet size and nuclear area
Lipid Droplets | Nucleus Lipid Droplets | Nuc Lipid Droplets | Nucleus used for combination shown in same image.

Studies Utilizing this Method/Kit
Study Start Date Study Types Description
Loading...