Method/Kit:
EarlyTox Cardiotoxicity Kit (Molecular Devices: R8211)

Supplier Molecular Devices
Measurement Type Fluorescence Intensity
Description The assay kit can be used for characterizing the impact of pharmacological compounds on peak frequency (BPM), peak amplitude, and beat pattern and is designed to work with stem cell-derived cardiomyocytes or primary cardiomyocytes. Peak frequencies are determined from changes in intracellular Ca++ concentration monitored by the EarlyTox Cardiotoxicity Dye. During a single beating cycle, Ca++ is released into the cytoplasm after the sarcoplasmic reticulum is stimulated. Calcium binds with troponin activating the sarcomere, and the cell contracts. Synchronously, there is an increase in fluorescent signal as dye binds to free calcium in the cytosol. Cellular relaxation occurs on removal of calcium from the cytosol by calcium uptake pumps of the sarcoplasmic reticulum and by calcium exchange with extracellular fluid. The fluorescent signal decreases as calcium concentration decreases. As the cycle repeats, additional fluorescent peaks synchronous with beating are observed.


Target/Analytes with this Method/Kit
Target/Analyte Short Name Description
Amplitude AMP Amplitude of calcium flux measured with EarlyTox Cardiotoxicity Kit. Measured in AFU (Arbitrary Fluorescence Units)
Beat Rate BEAT RATE Beat rate (e.g. of cardiomyocytes). Normally determined through video quantification.
Decay/Rise Ratio DECAY:RISE A ratio between rise (time to go from baseline fluorescence up to max fluorescence) and decay (time from max, back down to baseline) times for a spike in fluorescent signal (e.g., for a beat in a cardiomyocyte)
Decay Time Decay Time Average time of Ca2+ efflux in cell beating.
Rise Time Rise Time Average time to reach maximum Ca2+ influx in cell beating.

Studies Utilizing this Method/Kit
Study Start Date Study Types Description
Loading...