Method/Kit:
Immunocytochemistry and immunofluorescence protocol (DAPI, FITC, TRITC)

Measurement Type Imaging
Description DNA (DAPI, blue), Neutral Lipids (FITC, green), Mitochondria (TRITC, yellow)

Target/Analytes with this Method/Kit
Target/Analyte Short Name Description
Steatosis STEATOSIS Abnormal retention of lipids.
VE-Cadherin VE-Cadherin VE-cadherin belongs to the super-family of classical cadherins, and as such, mediates homotypic calcium-dependent cell–cell interactions.1 VE-cadherin expression is tissue-specific and exclusive to endothelial cells. Also know as Cadherin 5, type 2, or CD144 (Cluster of Differentiation 144). [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4049861/]
ZO-1 ZO-1 Recommended name: Tight junction protein ZO-1 Alternative name(s): Tight junction protein 1 Zona occludens protein 1 Zonula occludens protein 1 TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed:7798316, PubMed:9792688). [https://www.uniprot.org/uniprot/Q07157]
CD31 CD31 CD31, also known as platelet endothelial cell adhesion molecule-1 (PECAM-1), is a transmembrane homophilic receptor that is expressed by endothelial cells (ECs), platelets, granulocytes, macrophages, dendritic cells (DCs), T- and B-cells and natural killer (NK) cells. As a member of the immunoglobulin (Ig) gene superfamily, CD31 comprises six extracellular Ig folds; it has a molecular mass of 130 kDa and is differentially glycosylated, involving N-linked and O-linked glycosylation sites. [Federica M. Marelli-Berg, Marc Clement, Claudio Mauro, Giuseppina Caligiuri Journal of Cell Science 2013 126: 2343-2352; doi: 10.1242/jcs.124099 ]
Cell Morphology Cell Morphology Imaging, typically with transmitted light, to see the cell morphology.
Collagen IV a1,2 Col4A1,2 Collagen type IV alpha 1 and 2
Collagen IV a4 Col4A4 Collagen, type IV, alpha 4
DNA DNA Immunocytochemistry imaging.
Laminin Laminin Laminins are a major constituent of the basement membrane which is an intricate meshwork of proteins separating the epithelium, mesothelium, and endothelium from connective tissue. There are 15 different laminins, each consisting of a unique combination of three subchains. The combination of chains confers some tissue specificity. [D. Guldager Kring Rasmussen, M.A. Karsdal, in Biochemistry of Collagens, Laminins and Elastin, 2016]
Mitochondria Mitochondria Imaging of the mitochondria in live cells.
Nephrin Nephrin Quantification of Nephrin secretion into cell culture/supernatant
Neutral lipids Neutral lipids Neutral lipids observed in an image using LipidTox Green.
Nuclei Nuclei Nuclei as observed by image staining.
VE-Cadherin VE-Cadherin VE-cadherin belongs to the super-family of classical cadherins, and as such, mediates homotypic calcium-dependent cell–cell interactions.1 VE-cadherin expression is tissue-specific and exclusive to endothelial cells. Also know as Cadherin 5, type 2, or CD144 (Cluster of Differentiation 144). [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4049861/]
ZO-1 ZO-1 Recommended name: Tight junction protein ZO-1 Alternative name(s): Tight junction protein 1 Zona occludens protein 1 Zonula occludens protein 1 TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed:7798316, PubMed:9792688). [https://www.uniprot.org/uniprot/Q07157]

Studies Utilizing this Method/Kit
Study Start Date Study Types Description
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