Method/Kit:
Immunocytochemistry and immunofluorescence protocol (DAPI, FITC, Cy5)

Supplier Abcam
Measurement Type Imaging
Description Immunocytochemistry (Cy5, DAPI, FITC) used for imaging and observation of HO-1, DNA, OAT4.


Target/Analytes with this Method/Kit
Target/Analyte Short Name Description
Aquaporin 1 AQP1 Aquaporin 1
COL Ia1 COL Ia1 Fluorescent Expression of the COL1A1 Protein
Cytokeratin 8/18 CK 8/18 Human Hepatocyte specific cytokeratin intermediate filaments
FITC-Dextran FITCdex Direct read of FITC-Dextran in media effluent typically to assess permeability.
Image IMG Image for later analysis of intensity, morphology, distribution, location, etc.
OAT1 OAT1 Oat1 [Slc22a6, originally identified as NKT (novel kidney transporter)] plays a central role in the metabolism and handling of gut microbiome metabolites as well as putative uremic toxins of kidney disease. The organic anion transporter (OAT) subfamily has a role in handling of common drugs (antibiotics, antivirals, diuretics, nonsteroidal anti-inflammatory drugs), toxins (mercury, aristolochic acid), and nutrients (vitamins, flavonoids). [Nigam SK, Bush KT, Martovetsky G, et al. The organic anion transporter (OAT) family: a systems biology perspective. Physiol Rev. 2015;95(1):83-123. doi:10.1152/physrev.00025.2013]
OCT-2 Quantification (Imaging) OCT-2 Image based quantification of OCT-2 staining
octamer-binding transcription factor 4 Oct-4 Oct-4 is a homeodomain transcription factor of the POU family. It is critically involved in the self-renewal of undifferentiated embryonic stem cells.
SGLT2 SGLT2 Glucose transporter
ZO-1 ZO-1 Recommended name: Tight junction protein ZO-1 Alternative name(s): Tight junction protein 1 Zona occludens protein 1 Zonula occludens protein 1 TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed:7798316, PubMed:9792688). [https://www.uniprot.org/uniprot/Q07157]
BSEP Protein BSEP BSEP protein levels measured by immunoflourescence
CD31 CD31 CD31, also known as platelet endothelial cell adhesion molecule-1 (PECAM-1), is a transmembrane homophilic receptor that is expressed by endothelial cells (ECs), platelets, granulocytes, macrophages, dendritic cells (DCs), T- and B-cells and natural killer (NK) cells. As a member of the immunoglobulin (Ig) gene superfamily, CD31 comprises six extracellular Ig folds; it has a molecular mass of 130 kDa and is differentially glycosylated, involving N-linked and O-linked glycosylation sites. [Federica M. Marelli-Berg, Marc Clement, Claudio Mauro, Giuseppina Caligiuri Journal of Cell Science 2013 126: 2343-2352; doi: 10.1242/jcs.124099 ]
Cell Morphology Cell Morphology Imaging, typically with transmitted light, to see the cell morphology.
Collagen IV a1,2 Col4A1,2 Collagen type IV alpha 1 and 2
Collagen IV a1,2 | DNA | Laminin Col4A1,2|DNA|Laminin Multi-stain imaging target
Collagen IV a4 Col4A4 Collagen, type IV, alpha 4
Cytokeratin-18 CK-18 EMT marker.
Cytokeratin 19 CK-19 Cytokeratin 19
DNA DNA Immunocytochemistry imaging.
DNA | Albumin | ABCB11/BSEP DNA/ALB/BSEP ICC staining; imaging DAPI/FITC/CY5
DNA | Albumin | VE-Cadherin DNA|ALB|VEC ICC/IHC imaging targets
DNA | Albumin | ZO-1 DNA|ALB|ZO1 Imaging stain target
DNA | CYP3A4 | ZO-1 DNA|CYP3A4|ZO1 ICC/IHC imaging target
DNA | F-Actin | ABCB11/BSEP DNA | F-Actin | ABCB IHC/ICC imaging
DNA | F-Actin | alpha 1 Antitrypsin DNA | F-Actin | alph IHC/ICC imaging
DNA | F-Actin | CD68 DNA | F-Actin | CD68 ICC/IHC Imaging targets
DNA | F-Actin | E-Cadherin DNA|F-Actin|E-Cad IHC/ICC imaging targets
DNA | F-Actin | Human Albumin DNA | F-Actin | ALB IHC/ICC imaging
DNA | F-Actin | MDR1/ABCB1 DNA | F-Actin | MDR1 IHC/ICC imaging
DNA | F-Actin | MDR3 DNA | F-Actin | MDR3 IHC/ICC imaging
DNA | F-Actin | MRP1 DNA | F-Actin | MRP ICC/IHC imaging
DNA | F-Actin | MRP2 DNA | F-Actin | MRP2 IHC/ICC imaging
DNA | F-Actin | NTCP/SLC10A1 DNA | F-Actin | NTCP IHC/ICC imaging
DNA | F-Actin | OATP1B1 DNA | F-Actin | OAT ICC/IHC imaging targets
DNA | F-Actin | VE-Cadherin DNA | F-Actin | VE-C ICC/IHC Imaging targets
DNA | OAT1 DNA | OAT1 ICC / IHC imaging
DNA | OAT1 | ZO-1 DNA | OAT1 | ZO-1 Imaging of DNA, OAT1, and ZO-1
DNA | OAT1 | ZO-1 || DNA | CD31 | VE-Cadherin DNA|OAT1|ZO-1|CD31|V IHC / ICC imaging with different stains in different chambers Top (RPTEC): ZO-1 (green), OAT1 (red), DNA (blue) Bottom (HUVEC): CD31 (green), VE-cadherin (Red), DNA (blue)
DNA | OAT3 DNA | OAT3 ICC / IHC Imaging
DNA | OAT3 | ZO-1 DNA | OAT3 | ZO-1 ICC/IHC imaging
DNA | OAT4 | HO-1 Imm combo 3 Immunocytochemistry imaging.
DNA | OAT4 in Cells | HO-1 in Cells | Tissue Structure Imm combo 4 Immunocytochemistry imaging.
DNA | Occludin | CD68 DNA|Occludin|CD68 IHC/ICC imaging
DNA | OCT 2 DNA | OCT 2 ICC / IHC Imaging
DNA | OCT2 | ZO-1 DNA | OCT2 | ZO-1 Imaging of DNA, OCT2, and ZO-1
DNA | OCT2 | ZO-1 || DNA | CD31 | VE-cadherin DNA|OCT2|ZO1|CD31|VE ICC/ IHC zstack of the chip in the mimetas plate. Top (RPTEC): ZO-1 (green), OCT2 (red), DNA (blue) Bottom (HUVEC): CD31 (green), VE-cadherin (Red), DNA (blue)
DNA | ZO-1 DNA | ZO-1 Imaging of DNA and ZO-1
DNA | ZO-1 | b-tubulin DNA | ZO-1 | b-tubul Staining for DNA, ZO-1 and b-tubulin
DNA | ZO-1 | OAT3 || DNA | CD31 | VE-cadherin DNA|ZO1|OAT3|CD31|VE IHC/ICC zstack of the chip in the mimetas plate. Top (RPTEC): ZO-1 (green), OAT3 (red), DNA (blue) Bottom (HUVEC): CD31 (green), VE-cadherin (Red), DNA (blue)
HO-1 in Cells HO-1 Immunocytochemistry imaging.
Human Alkaline Phosphatase Human ALKP Human Alkaline Phosphatase
Image IMG Image for later analysis of intensity, morphology, distribution, location, etc.
Laminin Laminin Laminins are a major constituent of the basement membrane which is an intricate meshwork of proteins separating the epithelium, mesothelium, and endothelium from connective tissue. There are 15 different laminins, each consisting of a unique combination of three subchains. The combination of chains confers some tissue specificity. [D. Guldager Kring Rasmussen, M.A. Karsdal, in Biochemistry of Collagens, Laminins and Elastin, 2016]
Nephrin Nephrin Quantification of Nephrin secretion into cell culture/supernatant
Nuclei Nuclei Nuclei as observed by image staining.
Nucleus | Nephrin | CD34 N|Nephrin|CD34 Imaging target for the combined staining of nuclei, CD34, and nephrin
OAT1 OAT1 Oat1 [Slc22a6, originally identified as NKT (novel kidney transporter)] plays a central role in the metabolism and handling of gut microbiome metabolites as well as putative uremic toxins of kidney disease. The organic anion transporter (OAT) subfamily has a role in handling of common drugs (antibiotics, antivirals, diuretics, nonsteroidal anti-inflammatory drugs), toxins (mercury, aristolochic acid), and nutrients (vitamins, flavonoids). [Nigam SK, Bush KT, Martovetsky G, et al. The organic anion transporter (OAT) family: a systems biology perspective. Physiol Rev. 2015;95(1):83-123. doi:10.1152/physrev.00025.2013]
OAT4 in Cells OAT4 Immunocytochemistry imaging.
Tissue Structure Tissue Structure Tissue structure as observed by image staining.
VE-Cadherin VE-Cadherin VE-cadherin belongs to the super-family of classical cadherins, and as such, mediates homotypic calcium-dependent cell–cell interactions.1 VE-cadherin expression is tissue-specific and exclusive to endothelial cells. Also know as Cadherin 5, type 2, or CD144 (Cluster of Differentiation 144). [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4049861/]
Vimentin Vimentin EMT marker
ZO-1 ZO-1 Recommended name: Tight junction protein ZO-1 Alternative name(s): Tight junction protein 1 Zona occludens protein 1 Zonula occludens protein 1 TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed:7798316, PubMed:9792688). [https://www.uniprot.org/uniprot/Q07157]

Studies Utilizing this Method/Kit
Study Start Date Study Types Description
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