Method/Kit:
Cholesterol Assay Kit (Fluorometric Assay) - HDL and LDL/VLDL (ab65390)

Supplier Abcam
Measurement Type Fluorescence
Description Detection of FREE cholesterol and TOTAL cholesterol from two independent replicates from three different fractions of a single sample. Total sample, HDL fraction, and LDL/VLDL fraction; fractions separated by centrifugation with PRECIPITATION buffer.
Cholesterol esterase hydrolyzes cholesteryl ester to free cholesterol. OxiRed Probe detects all free cholesterol. FREE cholesterol samples don't get exposed to esterase.
Samples are measured by Fluorescence in a plate reader Ex/Em = 535/587 nm.
VLDL is calculated by subtracting the TOTAL cholesterol from the FREE cholesterol.

Target/Analytes with this Method/Kit
Target/Analyte Short Name Description
Very low-density lipoprotein (VLDL) VLDL Very low-density lipoprotein composed of cholesterol, triglycerides, and apoliproteins. Lipoprotein is made and released by hepatocytes.

Studies Utilizing this Method/Kit
Study Start Date Study Types Description
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