Method/Kit:
RT-PCR (Applied Biosystems: StepOnePlus and SYBR Green Reaction Mix)

Supplier Applied Biosystems
Measurement Type Fluorescence Intensity
Description Real-time PCR noted in Tuan work. RT-PCR (Applied Biosystems: StepOnePlus and SYBR Green Reaction Mix) Lin et al. Mol. Pharmaceutics 2014)

The SYBR Green I dye chemistry uses Applied Biosystems™ SYBR™ Green I dye, a highly specific, double-stranded DNA binding dye, to detect PCR product as it accumulates during PCR cycles. The most important difference between the TaqMan and SYBR Green I dye chemistries is that the SYBR Green I dye chemistry will detect all double-stranded DNA, including non-specific reaction products. A well-optimized reaction is therefore essential for accurate results.

Target/Analytes with this Method/Kit
Target/Analyte Short Name Description
ACN Expression normalized to 18S rRNA ACN Exp Aggrecan Expression by Real-time PCR. Indicates cartilage.
ADAMTS4 ADAMTS4 Cartilage degenerating enzyme, ADAM Metallopeptidase With Thrombospondin Type 1 Motif 4
ADAMTS5 ADAMTS5 ADAM Metallopeptidase With Thrombospondin Type 1 Motif 5, Cartilage degenerating enzyme
AGG AGG PCR target
Aggrecan ACAN Chondrogenic marker gene
ALP ALP PCR target
Bone sialoprotein 2 BSP2 Typical osteogenic markers
BSPII Expression normalized to 18S rRNA BSPII Exp BSPII Expression by Real-time PCR. Indicates bone.
COL2 COL2 PCR Target
Col2 Expression normalized to 18S rRNA Col2 Exp Col2 Expression by Real-time PCR. Indicates cartilage.
Collagen Type X COL10 Hypertrophy marker
Cyclooxygenase-2 COX2 The enzymes that produce prostaglandins
Gene Expression gene exp Use as the target when individual genes will be included as subtargets. Can be used for PCR and/or TempO-Seq, or RNA-Seq
IL-1β IL-1β Pro inflammatory cytokine
Interleukin-6 IL-6 Pro inflammatory cytokine
Matrix metallopeptidases-13 MMP-13 Hypertrophy marker
Matrix metalloproteinase-1 MMP-1 MMPs can degrade a wide variety of extracellular matrix components.
Matrix metalloproteinase-13 MMP13 MMPs can degrade a wide variety of extracellular matrix components.
Matrix metalloproteinase-2 MMP-2 MMPs can degrade a wide variety of extracellular matrix components.
Matrix metalloproteinase-3 MMP-3 MMPs can degrade a wide variety of extracellular matrix components.
Matrix metalloproteinase-9 MMP-9 MMPs can degrade a wide variety of extracellular matrix components.
MMP-13 Expression normalized to 18S rRNA MMP-13 Exp Real-time PCR. Catabolic gene.
MMP-1 Expression normalized to 18S rRNA MMP-1 Exp Real-time PCR. Catabolic gene.
MMP-3 Expression normalized to 18S rRNA MMP-3 Exp Real-time PCR. Catabolic gene.
Osteocalcin OCL Osteocalcin, also known as bone gamma-carboxyglutamic acid-containing protein (BGLAP), is a noncollagenous protein hormone found in bone and dentin. Because it has gla domains, its synthesis is vitamin K dependent. In humans, the osteocalcin is encoded by the BGLAP gene. Its receptor is GPRC6A.
Osteocalcin Expression normalized to 18S rRNA Osteocalcin Exp Osteocalcin Expression by Real-time PCR. Indicates bone.
RLP13A RLP13A PCR Target. Data provider indicates that "RPL13A is the endogenous control" (like 18S in other studies).
RUNX2 RUNX2 PCR Target
RUNX2 Expression normalized to 18S rRNA RUNX2 Exp RUNX2 Expression by Real-time PCR. Indicates bone.
SOX9 SOX9 PCR Target
SOX9 Expression normalized to 18S rRNA SOX9 Exp SOX9 Expression for Real-Time PCR. Indicates cartilage.
TGF-β3 TGF-β3 Transforming growth factor

Studies Utilizing this Method/Kit
Study Start Date Study Types Description