Method/Kit:
Flow Cytometry

Measurement Type Absorbance
Description Flow cytometry is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles.

Target/Analytes with this Method/Kit
Target/Analyte Short Name Description
All Cells/Single Cells AC/SC All Cells/Single Cells
All Cells/Single Cells/Non Viable Cells AC/SC/NVC AC/SC/NVC
All Cells/Single Cells/Viable Cells AC/SC/VC AC/SC/VC
All Cells/Single Cells/Viable Cells/CD13-/CD36-/CD41- CD16-/CD34+ CD38- HSCs V3 HSCs All Cells/Single Cells/Viable Cells/CD13-/CD36-/CD41- CD16-/CD34+ CD38- HSCs V3
All Cells/Single Cells/Viable Cells/CD13-/CD36+/CD71+ CD235+- Early Erythroid V3 Early Erythroid V3 All Cells/Single Cells/Viable Cells/CD13-/CD36+/CD71+ CD235+- Early Erythroid V3
All Cells/Single Cells/Viable Cells/CD13-/CD36-/CD71+- CD235+ Later Erythroid V3 Later Erythroid All Cells/Single Cells/Viable Cells/CD13-/CD36-/CD71+- CD235+ Later Erythroid V3
All Cells/Single Cells/Viable Cells/CD13-/CD41+ CD16-/CD36+- Megakaryocytes V3 Megakaryocytes All Cells/Single Cells/Viable Cells/CD13-/CD41+ CD16-/CD36+- Megakaryocytes V3
CD13- CD13- CD13-
CD13+ CD13+ CD13+
CD13+ CD235-/CD41- CD16- Early Granulocytes CD13+ CD235-/CD41-EG CD13+ CD235-/CD41- CD16- Early Granulocytes
CD13+ CD235-/CD41- CD16+ Late Granulocytes CD13+ CD235-/CD41- C CD13+ CD235-/CD41- CD16+ Late Granulocytes
CD13+ CD235-/CD41-/CD36+ CD16+- Late Monocytes CD13+ CD235-/CD41-/C CD13+ CD235-/CD41-/CD36+ CD16+- Late Monocytes
CD13-/CD36-/CD41- CD16-/CD34+ CD38- HSCs V3 CD13-/CD36-/CD41- CD CD13-/CD36-/CD41- CD16-/CD34+ CD38- HSCs V3
CD13-/CD36+/CD71+ CD235+- Early Erythroid V3 CD13-/CD36+/CD71+ CD CD13-/CD36+/CD71+ CD235+- Early Erythroid V3
CD13-/CD36-/CD71+- CD235+ Later Erythroid V3 CD13-/CD36-/CD71+- C CD13-/CD36-/CD71+- CD235+ Later Erythroid V3
CD13-/CD41+ CD16-/CD36+- Megakaryocytes V3 CD13-/CD41+ CD16-/CD CD13-/CD41+ CD16-/CD36+- Megakaryocytes V3
CD16- CD16- CD16-
CD16+ CD16+ CD16+
CD206 CD206 For flow cytometry, monocytes were stained with fluorescently labeled antibodies, including PE anti-human CD80 (Biolegend, 1:50), APC anti-human CD36 (Biolegend, 1:50), Pacific Blue™ anti-human CD206 (BD Pharmingen, 1:25) or Pacific Blue™ anti-human CD14 (Biolegend, 1:50), for 20 min on ice. The APC Mouse IgG2a (Biolegend, 1:50), PE Mouse IgG1(Biolegend, 1:50), and Pacific Blue™ Mouse IgG1 (Biolegend. 1:50) were used to stain the cells as isotype control, which was used to set the selected gate during testing. Then stained cells were collected by FACSCanto (BD Bioscience) and analyzed by FlowJo (Treestar, MAC version, v9.3.2) software.
CD235a- CD235a- Cells negative for CD235a
CD235a+ CD235a+ Cells positive for CD235a+
CD34- CD34- Cells negative for CD34
CD34+ CD34+ Cells positive for CD34
CD34+ CD38+ Lineage Differentiated Progenitors CD34+ CD38+ Lineage CD34+ CD38+ Lineage Differentiated Progenitors
CD36 CD36 For flow cytometry, monocytes were stained with fluorescently labeled antibodies, including PE anti-human CD80 (Biolegend, 1:50), APC anti-human CD36 (Biolegend, 1:50), Pacific Blue™ anti-human CD206 (BD Pharmingen, 1:25) or Pacific Blue™ anti-human CD14 (Biolegend, 1:50), for 20 min on ice. The APC Mouse IgG2a (Biolegend, 1:50), PE Mouse IgG1(Biolegend, 1:50), and Pacific Blue™ Mouse IgG1 (Biolegend. 1:50) were used to stain the cells as isotype control, which was used to set the selected gate during testing. Then stained cells were collected by FACSCanto (BD Bioscience) and analyzed by FlowJo (Treestar, MAC version, v9.3.2) software.
CD36- CD36- Cells negative for CD36
CD36+ CD36+ Cells positive for CD36
CD38- CD38- Cells negative for CD38
CD38+ CD38+ Cells positive for CD38
CD41a- CD41a- Cells negative for CD41a
CD41a+ CD41a+ Cells positive for CD41a
CD71- CD71- Cells negative for CD71
CD71+ CD71+ Cells positive for CD71
CD80 CD80 For flow cytometry, monocytes were stained with fluorescently labeled antibodies, including PE anti-human CD80 (Biolegend, 1:50), APC anti-human CD36 (Biolegend, 1:50), Pacific Blue™ anti-human CD206 (BD Pharmingen, 1:25) or Pacific Blue™ anti-human CD14 (Biolegend, 1:50), for 20 min on ice. The APC Mouse IgG2a (Biolegend, 1:50), PE Mouse IgG1(Biolegend, 1:50), and Pacific Blue™ Mouse IgG1 (Biolegend. 1:50) were used to stain the cells as isotype control, which was used to set the selected gate during testing. Then stained cells were collected by FACSCanto (BD Bioscience) and analyzed by FlowJo (Treestar, MAC version, v9.3.2) software.
EdU- EdU- Cells negative for EdU
EdU+ EdU+ Cells positive for EdU
Non-Viable Cells NON-VIABLE All Cells/Single Cells/Non Viable Cells | Count
Viable Cells VIABLE All Cells/Single Cells/Viable Cells | Count

Studies Utilizing this Method/Kit
Study Start Date Study Types Description
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