Method/Kit:
15V Stimulation

Measurement Type Electrical
Description Recording of the electrical response generated following a 15V electrical stimulation delivered through an exogenous field stimulating electrode.

Target/Analytes with this Method/Kit
Target/Analyte Short Name Description
Calcium Transients VALA Voltage analysis is performed using the CyteSeer image analysis software, which automatically recognizes Hoechst-stained nuclei, segments, and identifies each cell to quantify voltage data for each cell captured in the field of view. Cells are included if four voltage peaks are detected (first paced beat is only partially captured by the imaging system); cells with fewer than four peaks indicated inadequate capture, and cells with more than four peaks indicate that cells had a spontaneous beating rate that was faster than the paced beats. The peak rise time, CTD25 (25% duration of the calcium transient, or duration at 25% decline from maximum amplitude), CTD75 (75% duration of the calcium transient, or duration at 75% decline from maximum amplitude, T75-25 (time for voltage to decay from 75% to 25% of maximum), and downstroke velocity were quantified using the CyteSeer image analysis software.
Peak Count Peak Count The number of negative-going field potentials recorded in the spheroid of neuronal soma following electrical stimulation of the neurite outgrowth.
Voltage Peak Rise Time VPRT Voltage Peak Rise Time

Studies Utilizing this Method/Kit
Study Start Date Study Types Description
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