Method/Kit:
Vimentin Staining

Supplier Abcam
Measurement Type Fluorescence Intensity
Description Immunocytochemical staining for vimentin (3.7 μl/mL; ab92547)
Cells were fixed with 4% paraformaldehyde for 15 min, permeabilized with 0.5% Triton X for 10 min, and blocked with 3% bovine serum albumin in PBS for 30 min prior to incubation with primary antibodies overnight at 4°C. After washing with PBS 3 times 5 min each, devices were incubated with Alexa Fluor 488- and 594-conjugated secondary antibodies (Thermo Fisher Scientific) and diluted 1:1000 in 3% bovine serum albumin for 1 h in the dark. The devices were washed with PBS 3 times 5 min each, treated with NucBlue Live ReadyProbes Reagent (Thermo Fisher Scientific).

Target/Analytes with this Method/Kit
Target/Analyte Short Name Description
Vimentin Vimentin EMT marker
Vimentin Vimentin EMT marker

Studies Utilizing this Method/Kit
Study Start Date Study Types Description
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