Method/Kit:
Fluorescence (490/525)

Supplier None
Measurement Type Fluorescence Intensity
Description Direct read or quantification of FITC (normally)

Target/Analytes with this Method/Kit
Target/Analyte Short Name Description
(2R,3R,4S,5R)-3,4,5,6-Tetrahydroxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]hexanal 2-NBDG Fluorescent glucose analog
6-CF Uptake (cell lysate) 6-CF Uptake (cell) Uptake of 6-CF (OAT substrate), quantified in cell lysates through the use of a fluorescent plate reader.
CellTracker CellTracker Addition of Cell Tracker dye to ensure cell types are present.
Dextran-FITC (10kDa) FD10k Used as a tracer to determine cell tight gap formation/permeability.
EAM-1 Uptake (cell lysate) EAM-1 Uptake (cell) Uptake of EAM-1 (OCT substrate), quantified in cell lysates through the use of a fluorescent plate reader
FITC-Dextran [HUVEC] FITC-Dextran [HUVEC] Fluorescent readout from effluent in HUVEC channel. Used to determine permeability of HUVEC channel.
FITC-Dextran [RPTEC] FITC-Dextran [RPTEC] Fluorescence readout of FITC-dextran in RPTEC channel. Used to measure permeability
HUVEC Permeability (Fluorescence Imaging) Permeability-FITC Chip is treated with a fluorescent-tagged tracker molecule (like dextran), and imaged at various locations (ex. Channel + ECM). Permeability is determined based on fluorescence ratio between HUVEC and ECM.
Mitochondria Mitochondria Imaging of the mitochondria in live cells.
MRP2/4 Efflux MRP2/4 Measurement of transport activity via MRP2 and 4
Permeability 4kDa FITC-Dextran Permeability 4FD 4kDa FITC-Dextran measured in 485 (Ex)-528nm (Em)
Permeability (Fluorescence) Permeability (FLU) Permeability of a dye (measured by fluorescence) across a cell layer
P-gp P-gp Efflux transporter
Rhodamine 123 R123 Fluorescent P-gp substrate
RPTEC Permeability (Fluorescence Imaging) Permeability-FITC R Chip is treated with a fluorescent-tagged tracker molecule (dextran), and imaged at various locations (ex. RPTEC and ECM). Permeability is determined based on fluorescence ratio between RPTEC and ECM.
SGLT2 SGLT2 Glucose transporter
Transporter accumuation accumultion to measure protein transporter activity, the specific substrates are treated and then the transported substrates are measured
FITC-Dextran [HUVEC] FITC-Dextran [HUVEC] Fluorescent readout from effluent in HUVEC channel. Used to determine permeability of HUVEC channel.
FITC-Dextran [RPTEC] FITC-Dextran [RPTEC] Fluorescence readout of FITC-dextran in RPTEC channel. Used to measure permeability
Permeability (Fluorescence) Permeability (FLU) Permeability of a dye (measured by fluorescence) across a cell layer

Studies Utilizing this Method/Kit
Study Start Date Study Types Description
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