Method/Kit:
Thermo Scientific™ Pierce™ BCA Protein Assay Kit

Measurement Type Absorbance
Description Green-to-blue (A562nm), precise, detergent-compatible assay reagent to measure total protein concentration vs. protein standard.

Target/Analytes with this Method/Kit
Target/Analyte Short Name Description
Total Protein T.Protein Thermo Scientific™ Pierce BCA Protein Assay Kit remains one of the most popular protein quantitation methods worldwide. Used in more labs than any other detergent-compatible protein assay, Pierce BCA Reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity. Highlights: Colorimetric – estimate visually or measure with a standard spectrophotometer or plate reader (562nm) Excellent uniformity – exhibits less protein-to-protein variation than dye-binding methods Compatible – unaffected by typical concentrations of most ionic and nonionic detergents Moderately fast – much easier and four times faster than the classical Lowry method High linearity – linear working range for BSA equals 20 to 2000µg/mL Sensitive – detect down to 5µg/mL with the enhanced protocol BCA Protein Assay Applications: Studying protein:protein interactions Measuring column fractions after affinity chromatography Estimating percent recovery of membrane proteins from cell extracts High-throughput screening of fusion proteins This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Studies Utilizing this Method/Kit
Study Start Date Study Types Description
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