Method/Kit:
MTT (McAleer et al. 2019)

Measurement Type Absorbance
Description MTT powder was dissolved in growth medium to a final concentration of 5 mg/ml. Cells were incubated in 500 μl of the solution for 90 min at 37°C and 5% CO2. The medium was removed, and resultant crystals were dissolved in a lysis buffer consisting of 10% SDS with 0.5% acetic acid in dimethyl sulfoxide. The solution (100 μl) was placed into a 96-well plate and absorbance read at 570 nm using a BioTek Synergy HT plate reader (BioTek).

Target/Analytes with this Method/Kit
Target/Analyte Short Name Description
Cell Viability (Quantitative) Viability Measuring cell viability with a quantitative method like an LDH ELISA, or ATP with CellTiter-Glo

Studies Utilizing this Method/Kit
Study Start Date Study Types Description
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