Method/Kit:
HCS LipidTOX Red Neutral Lipid Stain (Thermo: H34476)

Supplier ThermoFisher
Measurement Type Fluorescence Intensity
Description The intracellular accumulation of neutral lipids, steatosis, is often triggered by drugs that affect the metabolism of fatty acids and/or neutral lipids. HCS LipidTOX™ Red neutral lipid stain was developed to characterize the potentially toxic effects of compounds on lipid metabolism in mammalian cell lines. The LipidTOX™ neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be detected by fluorescence microscopy or an HCS reader. This probe is compatible with the HCS LipidTOX™ phospholipidosis detection reagents (H34350, H34351). HCS LipidTOX™ neutral lipid stains can also be used to monitor the formation and differentiation of adipocytes, a process called adipogenesis.


Target/Analytes with this Method/Kit
Target/Analyte Short Name Description
Macrosteatosis Macrosteatosis Macrovesicular steatosis is the more common form of fatty degeneration and may be caused by oversupply of lipids due to obesity, obstructive sleep apnea (OSA),[8] insulin resistance, or alcoholism. (wikipedia)
macro-vesicular steatosis macro-vesicular steatotic vacuoles greater than 120 pixels
Microsteatosis Microsteatosis Microvesicular steatosis is characterized by small intracytoplasmic fat vacuoles (liposomes) which accumulate in the cell. Common causes are tetracyclines, acute fatty liver of pregnancy, Reye's syndrome, and hepatitis C. (wikipedia)
Micro-vesicular steatosis micro-vesicular stea steatotic vacuoles with pixel area less than 119

Studies Utilizing this Method/Kit
Study Start Date Study Types Description
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